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. 2014 Jun 20;9(6):e100253. doi: 10.1371/journal.pone.0100253

Figure 3. Immunoblotting analysis of surface-exposed proteins.

Figure 3

(A) Five recombinant SEPs purified from E. coli cell lysate were separated by 12% SDS-PAGE and stained by G-250 Coomassie Brilliant Blue. (B) Immunoblotting analysis of SEPs: lane M, protein molecular mass markers; lanes 1 to 5, rTolC, rPorin_4, rAdr1, rOmpW, and rAdr2.