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. 2014 Jun 4;137(7):1894–1906. doi: 10.1093/brain/awu114

Figure 6.

Figure 6

Treatment of cultured motor neurons with an endoplasmic reticulum stress inhibitor reduces endoplasmic reticulum stress-induced apoptosis. Purified embryonic primary wild-type (WT) and AR100 motor neuron were cultured for 7 days and were treated for 72 h with 50 nM dihydrotestosterone (DHT). Cultures were analysed by western blot to examine the levels of cleaved active caspase 12 in the absence and presence of 24 h salubrinal treatment. In the absence of salubrinal treatment there was a significantly higher level of active caspase 12 in AR100 motor neurons compared to the wild-type (n = 3, P = 0.003, t-test, n = number of replicate cultures). Following 24 h of salubrinal treatment, however, there was a significant decrease in caspase 12 expression in both wild-type and AR100 motor neurons (n = 3, P = 0.042 and 0.0004 respectively, t-test) and no longer a significant difference in expression between wild-type and AR100 motor neurons. Error bars show the SEM. *P < 0.05, **P < 0.01, ***P < 0.001. AU: arbitrary units.