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. 2014 Jun 20;9(6):e100062. doi: 10.1371/journal.pone.0100062

Table 2. Purification of recombinant QAPRTase from M. tuberculosis H37Rv.

Purification Step Total Protein (mg) Total activity (Unit) Yield (%) Specific activity (Unit/mg) Purification (fold)
Cell extract 481.8 91.5 100 0.2 1.0
His-Trap 34.3 62.7 68 1.8 9.6
Sephacryl S-200 14.0 17.1 19 1.2 6.4