Skip to main content
. 2014 Jun 20;9(6):e100000. doi: 10.1371/journal.pone.0100000

Figure 2. Quality controls on the antibody library.

Figure 2

(A) Representative dot-blot analysis of 94 supernatants of individual unselected library clones. The soluble scFv fragments in the bacterial supernatants were detected using an anti-myc-tag antibody (9E10) and a secondary antibody coupled with HRP. As positive control, a purified scFv fragment with a myc-tag was loaded (green circle). As a negative control, 2xYT medium was used (red circle). (B) A representative agarose gel of the PCR colony screening of unselected clones from the library, used primers capable of amplifying the scFv insert. As a negative control, non-transfected bacteria (left red arrow) and bacteria containing pHEN1 vector with a longer insert (right red arrow) were used. As a positive control, a phagemid vector with a scFv insert was used (green arrow). All six dot-blots and PCR-screening gels related to the library construction process are shown in Figure S2.