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. 2014 Jun 20;9(6):e100000. doi: 10.1371/journal.pone.0100000

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© 2014 Weber et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of the scFv and SIP antibody format. (B) Biacore characterization of the monomeric fraction of scFv (G5) produced at different concentrations on a CM5 Biacore microsensor chip, coated either with murine (left) or human recombinant BCD domains of tenascin-C (right). (C) SDS-PAGE of the G5 antibody in SIP format in reducing (R) and nonreducing (N) conditions, as well as gel-filtration profile on a S200 10/300 column. (D) Biacore profiles of the G5 antibody in SIP format, against murine and human recombinant BCD domains of tenascin-C.