Table 1. Primers used in qRT-PCR.
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
Spu-miR-31 | AGGCAAGATGTTGGCATAGCT | Qiagen miScript universal primer |
Spu-miR-2008 | ATCAGCCTCGCTGTCAATACG | |
Actr3 | CTACCATGTTCAGGGATTTCGG | GGTGAGAGATGACCTCTGTTTCG |
Clec4g | CCAACGGGAACCAAACAAT | TCGCAAACGCCAAACCTAAC |
Cndp2 | CTGTCGGATGGTTGGGATACTG | CATTCAGCCAGGCAAGAACG |
Coro6 | GCCATACGCATTCGTCATTTG | GATTGTTTCTCCTTCCTCCTCC |
Gnb2 | GCAAACATTTACCGGACACGAG | CAAGTAGCAAACGGCCACTCC |
H1f0 | CCATCGCCGACCTGAATG | AGCCTGAAGGTGCCACTCG |
Hnrnpa1 | GAGGTGATTATGCCAATGTCTGC | CACCACCGCCTCTTCCTTCT |
Hnrnpl | CACGCAAGTCATCCACAAAGTG | AAACATCCCTCCCATTCAGCC |
HSP90b1 | TACTCCGTTCTGGGTTCATGC | TCTTCCTCTTCTGGTTCCTCCTC |
Hspa8 | GTGCCAACCCATCATCACCA | ACCTGCACCTGGAAACCCTC |
Kiaa0196 | GTTGATTGGCGATACCAAGCAC | GACCTGTTCCCGCAGTTGAC |
Myo10 | GCCGTGGGTGCTAAGATGGT | GAGTTCTGCCTCCTGTGGACAAT |
Naca | AACAGTTCCGACCACAGGAAC | CTCTTCCAACCCACCATCATC |
Nnt | AGGTAAGCCTATGGCGATTGAG | CATCTCTGCGATTGGATACTGG |
Plekhd1 | AGGCCGAGGATTCCTTGTG | CTCTGCGGGAGATTGTGATGA |
Rpl12 | CTGTTGGTGGAGAAGTTGGAGC | CTGCCTGTTCTGGATGGTCAAC |
Slc25a5 | GCAGCTTACTTCGGGTTCTATG | GATCTTCCTCCAGCAGTCCAG |
Tgds | CTCAAGGCTCAATCACCAAGC | CGACCAAGGGAGGATGTGTT |
Yes1 | CGAAGATTCCGAATACACCGC | AGGACACCATAGGACCACACGT |