Figure 6. Viral RNA production.

MDBK cells were infected with wt BVDV p0, or BVDV R₁, R₂ or R₃ (MOI 1) and the intracellular viral RNA (ivRNA) was quantified at 24 h p.i. by real-time PCR. For each infection with BVDV R viruses, the results are expressed as ivRNA relative to wt BVDV p0. Mock-infected cells were added as control in each experiment. The results are shown as mean ± SD from two independent experiments in triplicate. *p<0.050; Student's t test vs wt BVDV p0.