Figure 7. Molecular characterization of BVDV R_1–5.

The complete nucleotide sequence of the Erns, Npro and NS region from wt BVDV p0, BVDV-TSC^r T_1–5 and BVDV R_1–5 genomes was obtained by direct sequencing. The nucleotide sequences are arranged in three or four codons and the position in the genome of the first nucleotide of each group of sequences is indicated above according to the complete genome of BVDV (GenBank accession no. NC_001461.1). Only the genome regions which showed mutations are displayed. Each BVDV-TSC^r T-R pair is shown in gray or white rows and, for non-synonymous mutations, amino acid changes are indicated below the corresponding nucleotide sequence, indicating the number of amino acids for each protein. Viruses that showed altered CPE (BVDV R₂, R₃ and R₅) are indicated in boldface.