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. 2014 Jun 20;9(6):e100546. doi: 10.1371/journal.pone.0100546

Figure 5. Changes in nuclear NF-κB levels 30 min post-TBS in the hippocampus.

Figure 5

Top panel: NF-κB was immunofluorescently labelled in organotypic hippocampal slice cultures. The p65 subunit of activated NF-κB was stained red using a Cy3 secondary antibody and nuclei were counterstained blue using Hoechst 33258. High magnification confocal images (40X) of the CA1, CA3 and dentate gyrus neuronal cell populations were captured for each slice. Scale bar = 50 µm. Bottom graphs: Levels of activated nuclear NF-κB fluorescence were measured in organotypic hippocampal slices 30 min after theta-burst stimulation (TBS). CA1, CA3 and dentate gyrus cell populations were compared for control and stimulated slices. There is an up-regulation in activated nuclear NF-κB in the CA1 region (*two-tailed Student's t tests, p value<0.05).