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. 2014 Jun 20;9(6):e100546. doi: 10.1371/journal.pone.0100546

Figure 6. Changes in nuclear NF-κB levels 30 min post-spatial learning in the hippocampus.

Figure 6

Top panel: Coronal section through the rat dorsal hippocampus (approx. −3.3 mm with respect to Bregma) immunofluorescently-labelled for the active form of the p65 subunit of NF-κB. Nuclei are stained red using propidium iodide and NF-κB labelled green using a FITC-conjugated secondary antibody. The white boxes represent the three regions of the hippocampal neuronal network that were analysed throughout this study. Scale bar = 400 µm. Middle panel: High-magnification images (40X) of the CA1, CA3 and DG illustrated by white boxes in the top montage. Scale bar = 50 µm. Bottom graphs: Levels of activated nuclear NF-κB fluorescence were measured in the rat hippocampus 30 min after water maze training ceased. CA1, CA3 and dentate gyrus cell populations were compared for passive control and trained rats. There is an up-regulation in activated nuclear NF-κB in the CA1 region (*two-tailed Student's t tests, p value<0.05).