Table 1. Histological grade, MIB1 labelling Index (LI) and 1p/19q ImmunoFISH and FISH status, by ratio and combination methods.

Patient #	WHO Grade	MIB1 LI (%)	Ratio								Combination %
			1p		Status		19q		Status		1p						Status		19q						Status
			Obs. #1	Obs. #2	ImFISH	FISH	Obs. #1	Obs. #2	ImFISH	FISH	obs. #1			obs. #2					obs. #1			obs. #2
											D	N	I	D	N	I	ImFISH	FISH	D	N	I	D	N	I	ImFISH	FISH
13	II	3	1.02	1.14	N/I	N	1.02	1.02	N	N	20	55	25	15	40	45	N	N	15	70	15	15	50	35	N	I *
33	II	5	0.44	0.47	D	D	0.42	0.49	D	D	92	3	5	88	5	7	D	D	98	2	0	88	7	5	D	D
16	II	6	0.38	0.68	D	D	0.48	0.54	D	D	75	15	10	62	18	20	D	D	80	15	5	70	17	13	D	D
23	II	6	1.13	1.16	I	N *	0.81	0.95	N	D *	12	57	31	22	35	43	N/I	N	37	52	11	28	47	25	N	D *
24	II	6	0.44	0.60	D	D	0.46	0.51	D	D	90	8	2	82	7	12	D	D	82	10	8	90	5	5	D	D
31	II	6	0.43	0.55	D	D	0.5	0.67	D	D	87	12	1	80	10	10	D	D	80	13	7	67	12	22	D	D
6	II	7	0.8	0.79	N	N	0.93	0.87	N	N	38	23	39	43	13	43	I	I	13	60	27	27	48	25	N	I *
14	II	9	0.81	0.84	N	N	0.88	0.87	N	N	46	28	26	43	22	35	I	I	23	37	40	28	17	55	I	I
30	II	9	0.64	0.93	D/N	D	0.82	0.74	D	D	28	49	23	30	40	30	N	D *	32	51	17	32	47	22	N	D *
28	II	10	0.54	0.63	D	D	0.54	0.58	D	D	73	10	17	62	12	27	D	D	67	18	15	65	12	23	D	D
20	II	11	0.55	0.53	D	D	0.53	0.51	D	D	83	10	7	83	7	10	D	D	83	12	5	88	7	5	D	D
9	III	5	1	0.94	N	N	0.98	1.11	N	N	13	58	29	29	38	32	N	I *	23	59	18	20	43	37	N	I *
27	III	5	0.9	1.20	N/I	D	0.95	0.93	N	D	30	60	10	22	39	39	N	D *	10	76	14	20	57	23	N	D *
18	III	6	0.99	1.09	N	I *	1.02	1.11	N/I	I	22	57	21	18	43	38	N	I *	20	59	21	13	63	23	N	I *
25	III	8	0.48	0.58	D	D	0.44	0.50	D	D	80	10	10	75	8	17	D	D	75	8	17	80	8	12	D	D
1	III	10	0.55	0.62	D	D	0.58	0.53	D	D	67	13	20	60	3	37	D	D	68	15	17	65	3	32	D	D
12	III	11	0.99	1.01	N	D *	0.96	0.97	N	N	8	69	23	20	47	33	N	I *	12	48	40	7	77	17	N	N
4	III	12	1.01	1.05	N	N	0.43	0.43	D	D	8	65	27	10	47	43	N	N	85	12	3	85	12	3	D	D
11	III	12	1.19	1.26	I	N	0.95	1.01	N	N	8	50	42	10	37	53	I	I	12	57	31	17	40	43	N	N
26	III	13	0.5	0.54	D	D	0.43	0.55	D	D	88	5	7	92	2	7	D	D	87	7	6	80	8	12	D	D
34	III	13	0.49	0.46	D	D	0.49	0.59	D	D	90	3	7	92	3	5	D	D	88	7	5	77	15	8	D	D
21	III	16	0.49	0.56	D	D	0.5	0.55	D	D	87	8	5	78	15	7	D	D	87	6	7	75	13	12	D	D
32	III	20	0.518	0.61	D	D	0.53	0.53	D	D	67	5	28	52	10	38	D	D	83	13	4	80	12	8	D	D
2	III	21	0.52	0.50	D	D	0.52	0.49	D	D	83	7	10	83	3	13	D	D	82	12	6	87	8	5	D	D
17	III	21	0.5	0.49	D	D	0.58	0.54	D	D	75	7	18	65	5	30	D	D	53	15	32	58	7	35	D	D
5	III	22	0.57	0.49	D	D	0.52	0.57	D	D	73	10	17	75	12	13	D	D	68	10	22	65	13	22	D	D
8	III	22	1.1	1.03	N	N	1.03	1.17	N/I	N	22	52	26	15	25	60	N/I	N	17	53	30	13	40	47	N	N
36	III	23	0.52	0.54	D	D	0.45	0.57	D	D	65	15	20	59	13	28	D	D	78	7	15	60	17	23	D	D
7	III	26	1.23	1.47	I	N *	1.05	1.01	N	N	8	32	60	17	28	55	I	I	1	47	52	15	22	63	I	I
35	III	29	0.33	0.38	D	D	0.38	0.42	D	D	94	3	3	93	3	3	D	D	83	7	10	87	2	12	D	D
15	III	34	0.84	0.89	N	N	0.65	0.70	D	D	37	37	26	28	22	50	I	I	57	28	15	42	15	43	I	I
22	III	34	0.57	0.51	D	D	0.45	0.60	D	D	74	13	13	75	3	22	D	D	78	5	17	53	15	32	D	D
29	III	34	0.52	0.53	D	D	0.45	0.59	D	D	65	10	25	60	17	23	D	D	77	5	18	65	10	25	D	D
10	IV	14	1.37	1.27	I	N *	1.03	1.09	N	N	8	43	49	17	37	47	I	I	13	54	33	12	33	55	N/I	I
19	IV	14	0.51	0.62	D	D	0.55	0.51	D	D	70	22	8	73	20	7	D	D	67	20	13	72	15	13	D	D
3	IV	19	1.05	1.16	N/I	N	0.54	0.49	D	D	18	47	35	13	37	50	N	N	83	10	7	92	3	5	D	D

D: deletion, N: no deletion and no imbalance, I: imbalance

*ImFISH/FISH discordance

ImFISH was calculated on 60 nuclei and FISH on 100.