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. 2004 Apr 1;4:6. doi: 10.1186/1472-6750-4-6

Figure 4.

Figure 4

Sandwich ELISA for virus detection. Double antibody sandwich ELISA absorbance values obtained in which dilutions of (A) AHSV or (B) BTV particles were trapped from suspension using virus-specific rabbit IgG and detected using phage-displayed scFvs (clone Lca12 for AHSV, clone H2 for BTV; see Figure 3) as the second antibody in the sandwich. Binding phages were detected with a pVIII-specific mAb and a peroxidase-labelled anti-mouse IgG immunoglobulin. Each point represents the average of three determinations. Error bars show one standard deviation from the mean.