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. 2014 Feb 19;23(14):3641–3656. doi: 10.1093/hmg/ddu073

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — HSP levels 20 h post-treatment with HSP990. Tissues were taken from 3- and 22-month-old mice 20 h after treatment with HSP990 (12 mg/kg) or vehicle. (A) ELISA of HSP70 levels in brain regions and peripheral tissues. Values were calculated relative to vehicle-treated young mice. (B) Relative protein level of HSP70, HSP40 and HSP25 in brain regions and peripheral tissues. Densitometric values were calculated relative to vehicle-treated young mice. (C) Representative western blots used for the quantification in (B). Data are the mean ± SEM. 5 < n < 6/group. **P < 0.01; ***P < 0.001. Asterisk indicates the statistically significant difference in the level of induction. Muscle, quadriceps; L.C., loading control; mth, months.