Figure 1.

Rbm20 is differentially expressed during cardiogenesis (A) Microarray roadmap of in vivo cardiogenesis. Illustration of mouse cardiac development stages from mESC through adult heart and dynamically and spatially differential expression patterns in the left and right ventricles (L: left: R: right). (B) In vitro differentiation of mESCs to EBs demonstrates that Rbm20 is expressed between d5 and d9 using quantitative polymerase chain reaction (qPCR). (C) qPCR analysis of established cardiac genes expressed during ESC differentiation to EBs at different time points. (D) Tnnt2-GFP reporter system in differentiating EBs shows the in vitro activation of troponin verifying in vitro cardiogenesis from d12 to d25. FACS analysis shows the fold change of cardiomyocyte population at d24 upon Zeo selection. *Relative to Eb d5 as a negative control P≤ 0.05.