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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: J Comp Neurol. 2010 Dec 15;518(24):4938–4962. doi: 10.1002/cne.22499

Figure 3.

Figure 3

Alternatively spliced neuroligin family members expressed during retinal development and relative abundance of gene expression. A: PCR primers flanking each of the conserved splice sites (sites A and B) for NLGN were used to detect NLGN isoform variation and give rise to several combinations of transcript variants. B: PCR amplification of cDNAs from a developmental time course of retinal tissues was separated by gel electrophoresis. C–E: Quantitative real-time PCR shows the relative abundance of NLGN1 (C), NLGN3 (D), and NLGN4 (E) transcripts in relation to the GAPDH housekeeping control gene. Error bars represent standard deviation (n = 3).