Table 1. Oligonucleotides Used for Cloning and Gene Analysis.
| Gene name | Accession #. | Forward primer | Reverse primer | Size (bp) |
|---|---|---|---|---|
| Cell markers & housekeeping | ||||
| GAPDH | NM_204305 | CCATTCCTCCACCTTTGATG | GGAACAGAACTGGCCTCTCA | 280 |
| Brn3 | NM_204759 | CCATCGCCGAGAAGTTAGAC | CAAACCCTGCCCAGTTCT | 208 |
| Lim1 | L35569 | AGTCCTCCGTTCTCCTCGATA | TCCCGAGGTGACATTGTCTT | 211 |
| Pax6 | D87837 | CACCACTTCCACAGGTCTCAT | TCGTGCCGAACCGATATAAT | 281 |
| Opsin (green) | NM_205490 | GAATTCTCCTCCCTGCTTCC | TTCTCCGTGCTGCTTCTGTT | 595 |
| Full coding sequences | ||||
| Neuroligin-1 | N/A | ATGGCCTTTCCAAGATCCA | CTACATTACCCTGGTTGTTGAGTGTGA | 2592,2565, 2532,2472 |
| Neuroligin-3 | N/A | ATGTGGCTGATACTCTGGAG | CTATACCCTGGTGGTGGAGTGTGAG | 2565,2499, 2439 |
| Neuroligin-4 | N/A | ATGTCGAAACCAATGGGACT | CTATACCCTAGTTGTTGAATGTCCA | 2541,2481, 2382 |
| Neuroligin – no splice site | ||||
| Neuroligin-1 | N/A | ACCCATGCACAAGAAGAGGA | TGGGCGTCATTAAGGGAATA | 169 |
| Neuroligin-3 | N/A | CGGTGGTGACAGCTGACCT | CTGTTGTAGGCGGGTGAGAT | 585 |
| Neuroligin-4 | N/A | CCCAGAGAAACACAACCAATG | GTATGCAAAGGCTGCATTCC | 245 |
| Splice site ‘A’ | ||||
| NL1-A site | N/A | ATCTATGTCCCAACCGAGGA | CCAGTGCCTTCCATGTAGGAC | 219,159,99 |
| NL3 –A site | N/A | CCCAACGAGGACTGTCTGTA | AGAAGGCGATGTTCTCACTG | 418,358,298 |
| NL4 –A site | N/A | ACCTGGATGAGAGGTCGTTG | AAGATCCGCCATGGATGTAG | 252, 192 |
| Splice site ‘B’ | ||||
| NL1- B site | N/A | CATGCGTCAATCTGTTGACTTT | GAGTGCTGTTCCACTTTGAGC | 113, 86 |
| NL3- B site | N/A | CATCACCGTCTTCGGTTCC | GGATGATGGCTCTCTGGAAG | 146 |
| NL4- B site | N/A | GATCTGGGGCAGGAGCTT | CGAAGGCATTCAACCAAATCAG | 202 |
| Neurexin | ||||
| NRXN1 | CGTAGTACGTTTCACGAGAAGTGG | AGACCAGAGAGCTGGCCTTG | 171, 261 | |
| NRXN3 | TGACAGTGCTCCTGGACTTG | TGATGTAGTTTCGCGTCTCG | 1511, 1268, 1208, 896, 887,704,326 |