Table 2. Information on Primary Antibodies Used in This Study (See Also Materials and Methods).
| Antibody | Immunogen used | Source | Dilution IHC | Dilution Western |
|---|---|---|---|---|
| Calbindin-D281 | Full-length calbindin- purified from chicken gut | Sigma, mouse monoclonal, CL3002 | 1:500 or 1:100,0001 | 1:1,000 |
| Calretinin | Full-length recombinant human protein | SWANT, rabbit polyclonal, 7699/4 | 1:1,000 | |
| CASK | Amino acids 318-415 of rat CASK/Lin2 | UC Davis NeuroMab Facility, mouse monoclonal, clone K56A/50 | 1:200 | 1:200 or 1:1,000 |
| GABA | GABA conjugated to bovine serum albumin | Sigma, rabbit polyclonal, A2052 | 1:2,500 | |
| GluR4 | Peptide sequence RQSSGLAVIASDLP of rat GluR4 | Chemicon, rabbit polyclonal, AB1508 | 1:500 | N/A |
| Hemagluttinin (HA-tag) | HA peptide sequence [YPYDVPDYA] from the human hemagglutinin protein. | Roche, rat monoclonal, 1-867-423 clone 3F10 | N/A | 1:1,000 |
| Islet-1 | Amino acids 247-349 at the C-terminus of Islet1 | DSHB, mouse monoclonal, 40.2D6 | 1:35 | N/A |
| LIM 1+2 | Amino acid residues 1-360 of rat Lim2 protein | DSHB, mouse monoclonal, 4F2 | 1:20 | N/A |
| LIM3 | Recombinant full- length murine Lim3 fused to GST | DSHB, mouse monoclonal, 67.4E12 | 1:50 | 1:50 |
| Neuroligin-11 | Intracellular C-terminal domain (aa 718-843) of rat NLGN1 | UC Davis NeuroMab Facility, mouse monoclonal, N97/A31- or N97/A31-purified | 1:10 1:500 purified or 1:5,0001 | 1:500 purified |
| Pax6 | Peptide QVPGSEPDMSQYWPRLQ from the C-terminus of mouse Pax6 | Covance, rabbit polyclonal, PRB-278P | 1:8,000 | N/A |
| Pax6 | Amino acids 1–223 of chicken Pax6 | DSHB, mouse monoclonal, Pax6-concentrate | 1:200 | N/A |
| Prox1 | C-terminal homeo- and Prospero domains (aa 546-736) of human Prox1. | Chemicon, rabbit polyclonal, AB5475 | 1:10,000 | N/A |
| PSD95 | Amino acids 77-299 of the human PSD95. | 75-028 UC Davis NeuroMab Facility, mouse monoclonal, clone K28/43 | 1:1,000 - 1,1500 | 1:2,000 |
| Ribeye (CtBP2/ribeye) | Amino acids 361-445 from the C-terminal region of mouse CtBP2. | BD-Biosciences, mouse monoclonal, 612044 | 1,000-1,2000 | N/A |
| TrkA | Extracellular domain of chicken trkA purified as a secreted protein from transfected COS cells by chroma- tography on wheat germ agglutinin, a His-Bind nickel-chelating column, and ion-exchange chromatography on Mono Q. | Dr. F. Lefcort, Montana State University, rabbit polyclonal | 1:6,000 | N/A |
| Gα t1 (K-20; transducin) | Gα t1 of human origin corresponding to amino acids 90-109. | SantaCruz, rabbit polyclonal, sc-389 | 1:1,000 | N/A |
| SV2 | From purified cholinergic synaptic vesicles from the electric organ of Discopyge ommato. | DSHB, mouse monoclonal, SV2 | 1:50-1:100 | N/A |
Mouse monoclonal, M; rat monoclonal, Mr; rabbit polyclonal, R.
Hybridoma supernatatnt; IHC, immunohistochemistry.
1
Tyramide signal amplification (TSA).
2
No longer available (but available through SWANT). DSHB, developmental studies hybridoma bank; N/A, not applicable.