Figure 1. The N-terminal region is required for nuclear localization of Jmjd3 (A) Schematic presentation of Jmjd3 domain structure, including the catalytic JmjC domain. HEK cells were transfected with expression vectors for Flag-tagged full-length (FL), C-terminal domain (C-term), and N-terminal domain (N-term) of Jmjd3. (B) The expression of Jmjd3 full-length and deletion mutants was confirmed by western blot using anti-Flag antibodies. (C) The subcellular localization of these proteins was observed with immunofluorescence with anti-Flag antibodies (red). Cell nuclei were stained with DAPI (blue). (D) Quantification of the transfected cells showing the nuclear accumulation of Jmjd3 recombinant proteins. The values are shown as the mean of three independent experiments. n = total numbers of counted cells. Error bars equal one standard deviation from the mean. Statistics were performed using the Student’s t test analysis.