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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Genesis. 2014 Mar 14;52(6):581–587. doi: 10.1002/dvg.22762

Figure 2. Reversed gut/gonad orientation is independent of L/R asymmetric expression of gcy-5 in the ASER neuron.

Figure 2

a) Normal (dextral) male cultured at 20°C expressing gcy-5p::GFP on the right. b) Male cultured at 25°C with reversed gut/gonad asymmetry expressing gcy-5p::GFP. Note that gcy-5 is expressed on the right as with the animal with normal gut/gonad orientation. c) gpa-16(it143) I; gcy-5p::GFP V male cultured at 25°C with situs inversus totalis. gcy-5 is expressed on the left, owing to the complete mirror-image anatomical handedness. d) Normal (dextral) hermaphrodite cultured at 20°C expressing gcy-5p::GFP on the right. e) gpa-16(it143) I; gcy-5p::GFP V hermaphrodite grown at 25°C showing sinistral anatomical handedness and gcy-5 expression on the left. All animals are presented with ventral side up and anterior to left. Arrowhead indicates position of the gcy-5-expressing neuron. Asterisk indicates position of the gonad.