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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Microbiol. 2014 Feb 13;16(7):1094–1104. doi: 10.1111/cmi.12263

Fig. 11. Effects of C12 on cytosol/mitochondria cytochrome C in WT and DKO MEF: ELISA assays.

Fig. 11

WT and DKO MEF grown on plastic dishes were incubated with Ringer’s (control) or Ringer’s + 50 μM C12 (C12) for 1 hr followed by preparation of cytosolic and mitochondrial fractions and assays for cytochrome C (by ELISA) and VDAC and actin (by western blot). A. C12 increased cytosolic/mitochondrial cytochrome C ratio for WT and DKO MEF. Average+/−SD, n = 3 separate samples. *p<0.05 for C12 vs. control. B. Western blots showed VDAC in mitochondrial but not cytosolic fractions; actin was present prominently in cytosolic and less in mitochondrial fractions. Results typical of two similar experiments.