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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Microbiol. 2014 Feb 13;16(7):1094–1104. doi: 10.1111/cmi.12263

Fig. 5. Effects of C12 on caspase 3/7 in WT and DKO MEF, and the (lack of) effect of Bak and Bax expression.

Fig. 5

WT and DKO MEF stably expressing GFP, Bak or Bax were treated with either 50 μM C12 or 10 μM staurosporine for 2 hrs, followed by measurement of caspase 3/7 activity. C12 activated caspase 3/7 in WT and in DKO MEF, whether or not they expressed GFP or Bak or Bax. Staurosporine activated caspase 3/7 in WT and in DKO MEF that expressed Bak or Bax, but not in DKO (parental) or DKO+GFP MEF. Caspase activities in RLU were normalized in each comparison to values observed in the absence of C12 (control). Data are avg +/− SD (n = 3 expts). * p< 0.05 for comparisons between C12-treated or staurosporine-treated vs. control.