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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Microbiol. 2014 Feb 13;16(7):1094–1104. doi: 10.1111/cmi.12263

Fig. 9. Effects of C12 on cytochrome C and nuclei in WT and DKO MEF.

Fig. 9

WT and DKO MEF grown on coverslips were incubated with Ringer’s (control) or Ringer’s + 50 μM C12 (C12) for 1 hr followed by fixation, staining (Hoechst 33342 for nuclei and anti-cytochrome C) and confocal imaging of cytochrome C and Hoechst 33342. Arrows point to cytochrome C staining in the nuclei in C12-treated cells. Arrowheads point to nuclei that were shrunken and fragmented in C12-treated cells. Images are typical of >100 cells each in three similar experiments.