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. 2014 Apr 7;4(6):1081–1089. doi: 10.1534/g3.114.010819

Figure 2.

Figure 2

Loss-of hlh-17 affects extracellular DA levels. (A) mRNA levels in L4-stage hlh-17(ns204) animals when normalized against mRNA levels in age-matched wild-type animals. Light gray shading represents wild-type range of expression (1.0 ± 0.115). The levels of cat-1 and mod-5 mRNA are not significantly affected in hlh-17(ns204) animals. (B) hlh-17(ns204) animals demonstrate SWIP behavior that is an intermediate of the behavior in N2 and dat-1(ok157) animals, and that is rescued by transgenic expression of HLH-17::GFP. The bar for hlh-17R represents the average measurements from three biological replicas of three independent lines. For all strains except hlh-17R, n = 30 animals/rep/strain. For hlh-17R, n was equal to an average of at least 15 animals/line/biological rep (range, 12–26) because of differences in transmission frequency of the transgene. (C) SWIP phenotype in double mutant hlh-17(ns204); dat-1(ok157) and hlh-17(ns204); dop-3(vs106) animals is more similar to the phenotype in dat-1(ok157) and dop-3(vs106) animals, respectively, than in wild-type animals. The SWIP phenotype of hlh-17(ns204); dat-1(ok157); dop-3(vs106) animals is not significantly different from the SWIP phenotypes of dop-3 or hlh-17(ns204); dop-3(vs106) animals. n = 30 animals/rep/strain for three biological replicas. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001.