Figure 1.

Characterization of ADAM10 transgenic mice. Immunohistochemical detection of HA-tagged ADAM10 using the antibody Y-11. Cortex (A) and hippocampal (B) sections of ADAM10-mo transgenic animals and a nontransgenic control (FVB/N) are shown. Scale bars: 200 ∝m in A and 100 ∝m in B. (C) Quantitation of catalytically active ADAM10 protein levels. Enriched ADAM10 proteins from adult mouse brains were subjected to Western blot analysis and detected with an anti-ADAM10 antibody. The upper panel shows mature ADAM10 (<m) and the proform of ADAM10 (<p). The mature 62-kDa ADAM10 form was quantified using a ³⁵S-labeled secondary antibody (lower panel).