Fig. 4.

Novel allele at Badh 2 locus. A. A single nucleotide substitution was identified at exon-splicing junction, G to A, which is Kpn I resctriction enzyme recogniton site. Jakou-ine in Clade 8 carried the mutation type and reference genome Nipponbare, non-fragrant elite varieties carried a general type. B. PCR amplicon in Nioi-mochi 2 was not digested by enzyme digestion but others were. All fragrant landraces were applied and only six landraces in Clade 8 showed that their amplicons were not able to digest.