Table 1.
MSAP band type | H | M | Huyou 2 | Fengyou 1 | ||||
---|---|---|---|---|---|---|---|---|
|
|
|||||||
CK | 45°C | 37–45°C | CK | 45°C | 37–45°C | |||
Type I | 1 | 1 | 479 | 479 | 479 | 574 | 494 | 497 |
Type II | 1 | 0 | 58 | 61 | 60 | 65 | 72 | 74 |
Type III | 0 | 1 | 138 | 138 | 140 | 145 | 223 | 223 |
Total amplified bands | 675 | 678 | 679 | 784 | 789 | 794 | ||
Total methylated bands | 196 | 199 | 200 | 210 | 295 | 297 | ||
MSAP (%) | 29.0 | 29.4 | 29.5 | 26.8 | 37.4 | 37.4 | ||
Fully methylated ratio (%) | 20.4 | 20.4 | 20.6 | 18.5 | 28.3 | 28.1 |
CK indicates the control check.
A score of 1 and 0 represents the presence and absence of bands, respectively.
H and M represent digestion with HpaII/EcoRI and MspI/EcoRI, respectively.