Table 2.
Changes of DNA methylation pattern under control and heat stress in Huyou 2 and Fengyou 1
| Banding pattern | CK | T | Methylation status | 45°C | 37–45°C | |||||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
|
|||||||
| H | M | H | M | Huyou 2 | Fengyou 1 | Huyou 2 | Fengyou 1 | |||
| A1 | 0 | 0 | 1 | 1 | CCGG GGCC |
CCGG GGCC |
2 | 5 | 4 | 2 |
| A2 | 0 | 1 | 1 | 1 |
CCGG GGCC |
CCGG GGCC |
1 | 0 | 0 | 1 |
| A3 | 1 | 0 | 1 | 1 |
CCGG GGCC |
CCGG GGCC |
0 | 0 | 2 | 0 |
| A4 | 0 | 0 | 1 | 0 |
CCGG GGCC |
CCGG GGCC |
2 | 6 | 2 | 9 |
| A5 | 0 | 0 | 0 | 1 | CCGG GGCC |
CCGG GGCC |
1 | 4 | 3 | 4 |
| Demethylation | 6 (0.9%) | 15 (1.9%) | 11 (1.6%) | 16 (2.0%) | ||||||
| B1 | 1 | 1 | 1 | 0 | CCGG GGCC |
CCGG GGCC |
1 | 1 | 1 | 0 |
| B2 | 1 | 1 | 0 | 1 | CCGG GGCC |
CCGG GGCC |
1 | 74 | 0 | 75 |
| B3 | 1 | 1 | 0 | 0 | CCGG GGCC |
CCGG GGCC |
1 | 11 | 2 | 12 |
| B4 | 0 | 1 | 0 | 0 | CCGG GGCC |
CCGG GGCC |
1 | 0 | 1 | 0 |
| Methylation | 4 (0.6%) | 86 (10.7%) | 4 (0.6%) | 87 (10.9%) | ||||||
| C1 | 1 | 1 | 1 | 1 | CCGG GGCC |
CCGG GGCC |
476 | 489 | 476 | 488 |
| C2 | 1 | 0 | 1 | 0 |
CCGG GGCC |
CCGG GGCC |
58 | 66 | 57 | 65 |
| C3 | 0 | 1 | 0 | 1 | CCGG GGCC |
CCGG GGCC |
136 | 145 | 137 | 145 |
| No change | 670 (98.5%) | 700 (87.4%) | 670 (97.8%) | 698 (87.1%) | ||||||
CK and T indicate the control and heat stress conditions, respectively. Methylated cytosine is underlined, A score of 1 and 0 represents the presence and absence of bands, respectively. H and M represent digestion with HpaII/EcoRI and MspI/EcoRI, respectively.