Table II.
Key parameters for candidate planar cell expansion technologies
| Type | Name | Surface area, ai (cm2) | Consumables unit price, pconsum ($) | Media req., Vmedia (mL/cm2) | Labor requirements (time per operator to handle max # units) | Requires biosafety cabinet | Incubator capacity, Uincub (# units) | Ancillary control and automation equipment | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Seed time, tseed (h) | Feed time, tfeed (h) | Harvest time, tharvest (h) | Max # unitsa | Capacity, Uanc (# units) | Price, panc ($) | |||||||
| T-flasks | T175 | 175 | 9 | 0.25 | 0.38 | 0.38 | 0.75 | 10 | Y | 100 | — | — |
| T225 | 225 | 10 | 0.25 | 0.38 | 0.38 | 0.75 | 10 | Y | 100 | — | — | |
| T500 | 500 | 15 | 0.40 | 0.38 | 0.38 | 0.75 | 10 | Y | 100 | — | — | |
| Multi-layersb | L-1 | 636 | 60 | 0.25 | 0.15 | 0.15 | 0.30 | 1 | Y | 60 | — | — |
| L-2 | 1,272 | 73 | 0.25 | 0.15 | 0.15 | 0.30 | 1 | Y | 60 | — | — | |
| L-5 | 3,180 | 241 | 0.25 | 0.20 | 0.20 | 0.40 | 1 | Y | 24 | — | — | |
| L-10 | 6,360 | 507 | 0.25 | 0.25 | 0.25 | 0.50 | 1 | N | 12 | — | — | |
| L-40 (aut) | 25,440 | 1,265 | 0.25 | 0.08 | 0.08 | 0.17 | 4 | N | 16b | 16 | 425,000 | |
| Compact flasksd | cT | 1,720 | 19 | 0.33 | 0.38 | 0.38 | 0.75 | 10 | Y | 100 | — | — |
| Compact multi-layerse | cL-12 | 6,000 | 575 | 0.22 | 0.20 | 0.20 | 0.40 | 1 | N | 24 | — | — |
| cL-36 | 18,000 | 1,050 | 0.22 | 0.25 | 0.25 | 0.50 | 1 | N | 12 | — | — | |
| cL-120 (aut) | 60,000 | 3,000f | 0.20 | 0.08 | 0.08 | 0.17 | 4 | N | 16c | 16 | 425,000 | |
| Multi-layer bioreactorsg | bL-10 | 6,360 | 2,506 | 0.27 | 0.75 | 0.25 | 0.50 | 1 | N | 6 | 1 | 56,000 |
| bL-50 | 31,800 | 5,586 | 0.19 | 0.75 | 0.25 | 0.50 | 1 | N | 4 | 1 | 56,000 | |
| bL-180 | 114,480 | 13,986 | 0.17 | 0.75 | 0.25 | 0.75 | 1 | N | 2 | 1 | 56,000 | |
| Hollow fiber bioreactorsh | HF | 21,000 | 12,000 | 0.37 | 0.20 | 0 | 0.20 | 1 | N | — | 1 | 150,000 |
a
Max # units = Maximum number of units that can be handled by one operator simultaneously.
b
For example, Cell Factory systems (Nunc), CellSTACK (Corning).
c
It is assumed that L-40 and cL-120 use a specific incubator, while the other systems use a typical double-stack incubator.
d
For example, HYPERFlask (Corning).
e
For example, HYPERStack (Corning).
f
Price of cL-120 not available, calculated based on cL-36 price ($25/layer).
g
For example, Integrity Xpansion (ATMI).
h
For example, Quantum (TerumoBCT).