Figure 1.
Proliferation features of dental pulp stem cells ( DPSC s) treated by 17β‐oestradiol. (a) Isolated DPSCs were spindle‐like cells with a positive staining for STRO‐1 by immunocytochemistry. (b) PBS served as a negative control. (c) High concentration (10−5 m) of 17β‐oestradiol (E2) had negative effects on the proliferation of DPSCs, while the physiological concentrations (10−7 and 10−9 m) of E2 did not affect the cell growth. (d) MTT assay showed that 10−7 m E2 had no effects on the proliferation of DPSCs in vitro.