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. 2014 Mar 4;92(2):258–272. doi: 10.1111/mmi.12549

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© 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — FlaJ is not required for flagellin glycosylation but is required for export.A. Motility of A. caviae wild-type, flaJ mutant, flaJ mutant harbouring pSRK flaA, and flaJ mutant harbouring pSRK_hisflaA inoculated on 0.25% semisolid bacteriological agar.B. Western blots of FlaA/B(+pse) using α-FlaA/B(+pse) antibodies of whole-cell preparations and secreted fractions of A. caviae strains (wild-type, wild-type + pSRK, wild-type + pSRK_hisflaA, maf1 mutant, flaJ mutant, flaJ mutant + pSRK, flaJ mutant + pSRK_flaA, and flaJ mutant + pSRK_hisflaA). Coomassie-stained SDS-PAGE gels showing whole-cell preparations and secreted fractions are given as a loading control. The samples were also probed with α-GroEL as a cell lysis control for secreted fractions and an additional loading control for whole-cell fractions.C. Western blots of FlaA/B(+pse) using α-FlaA/B(+pse) antibodies of whole-cell preparations and secreted fractions of A. caviae strains (wild-type, wild-type + pSRK, wild-type + pSRK_flaA-CBD), Coomassie-stained SDS-PAGE gels showing whole-cell preparations and secreted fractions are given as a loading control. The samples were also probed with α-GroEL as a cell lysis control for secreted fractions and an additional loading control for whole-cell fractions.D. Western blots of FlaA/B(+pse) using α-FlaA/B(+pse) antibodies of whole-cell (WC) preparations and secreted fractions (SN) of A. caviae wild-type and flaH mutant strains. A Coomassie-stained SDS-PAGE gel showing whole-cell preparations and secreted fractions is given as a loading control. The samples were also probed with α-GroEL as a cell lysis control for secreted fractions and an additional loading control for whole-cell fractions.