Table 2.
Effects of WIN 55,212-2 mesylate in combinations with four classical antiepileptic drugs on learning, muscular strength and motor performance in mice
| Treatment (mg/kg) | Retention time (s) | Grip-strength (N) | Motor coordination impairment (%) |
|---|---|---|---|
| Vehicle | 180 (180; 180) | 0.92 ± 0.04 | 0 |
| WIN (5.0) + vehicle | 173.5 (155; 180) | 0.91 ± 0.04 | 25 |
| Clobazam (1.58) + WIN (5.0) | 180 (180; 180) | 0.90 ± 0.05 | 12.5 |
| Clonazepam (0.0022) + WIN (5.0) | 180 (180; 180) | 0.91 ± 0.05 | 0 |
| Phenobarbital (6.49) + WIN (5.0) | 180 (180; 180) | 0.90 ± 0.04 | 12.5 |
| Valproate (62.11) + WIN (5.0) | 180 (155.8; 180) | 0.90 ± 0.05 | 25 |
Results are presented as: (1) median retention times (in seconds; with 25th and 75th percentiles in parentheses) from the passive avoidance task, assessing learning in mice; (2) mean grip-strengths (in Newtons ± SEM) from the grip-strength test, assessing skeletal muscular strength in mice; and (3) percentage of animals showing motor coordination impairment in the chimney test in mice. Each experimental group consisted of eight mice. Statistical analysis of the data from the passive avoidance task was performed with non-parametric Kruskal–Wallis ANOVA test. Results from the grip-strength test were analyzed with one-way ANOVA. The Fisher’s exact probability test was used to analyze the results from the chimney test. All drugs were administered i.p. at specific pretreatment times scheduled from the 6 Hz-induced psychomotor seizures and at doses corresponding to their ED50 values against 6 Hz-induced convulsions in mice (for more details see the legend to Table 1)