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. 2004 May 15;113(10):1447–1455. doi: 10.1172/JCI21227

Figure 6.

Figure 6

CSD altered the immunostaining of laminin, ZO-1, and EBA within the blood vessel wall. (A_C) Laminin (approximate molecular weight, 200 kDa), a basement membrane protein, was reduced ipsilateral to CSD on immunoblots (at 12 hours) compared with that of the sham group and compared with that of the contralateral side. Similarly, CSD decreased the immunostaining of laminin in blood vessels (C), indicating that CSD alters the antigenicity of an integral basement membrane protein (scale bars: 100 ∝m). (B) These images show colocalization of the tight junction protein ZO-1 with RECA and show that CSD decreases the extent of this colocalization (colocalization coefficient, 0.21 ± 0.03 for CSD versus 0.55 ± 0.05 for control; P < 0.05). Compared with the image of the opposite side (nCSD), the merged image after CSD shows large segments of blood vessels with green fluorescence only (arrows; scale bars: 20 ∝m). (C) EBA immunostaining was also reduced in cortical blood vessels after CSD. This decrease often indicates impaired BBB function (scale bars: 100 ∝m).