Figure 3.

Figure 3A. Quantification of Ad5.SSTR/TK.RGD vector in ascites samples. The Ad5.SSTR/TK.RGD genome copies were quantified in each patient’s ascites sample (in triplicate) with primers and probe specific for RGD-4C-coding sequence (RGD) and then, normalized to amount of cellular DNA detected in the same sample with primers and probe for human μ-actin (housekeeping gene) using duplexing quantitative PCR settings. No RGD DNA was detected pretreatment (data not depicted in figure).

Figure 3B. Assessment of vector-mediated expression of HSV1-tk gene in ascites samples. HSV1-tk gene-specifc mRNA was quantified using total mRNA isolated from ascites samples as template (in triplicate) and then, normalized to the amount of human GAPDH (housekeeping gene) mRNA, which was detected in the same sample using duplexing RT-PCR settings. No TK mRNA was detected pretreatment (data not depicted in figure).

Figure 3C. Assessment of vector-mediated expression of SSTr2 gene in ascites samples. SSTr2 gene-specific mRNA was quantified using total mRNA isolated from each ascites sample as template (in triplicate) for reverse transcription reaction followed by quantitative PCR. The determined copy number of SSTr2 mRNA was normalized to the amount of human GAPDH (housekeeping gene) mRNA, which was detected in the same sample using duplexing RT-PCR settings.