Figure 5.

Success rate of meganuclease design methods. (Left) Experimental results on targets sampled from ETS (extended target space). (Right) Experimental results on targets sampled from RTS (restricted target space). SeqMact - machine learning predictions, SeqMact + — machine learning predictions with additional I132V mutation, Comb — combinatorial libraries. GTAC — proportion of GTAC target variants with at least one positive mutant, ORIG — proportion of original (sampled) targets with at least one positive mutant, ORIGstrong — proportion of original (sampled) targets with at least one highly active mutant (normalized cleavage activity score above 0.8).