Table 1.

Reduced Proteins in HIV-1 Derived from CEMX174 Cells Following Virion Purification using DM or Optiprep

% of sequence coverage	accession number	protein name	peptides (95%)	DM:control	P-value	Optiprep: control	P-value
42.9	P63104	14-3-3 protein zeta/delta	6	0.5151	0.0371	0.7533	0.0613
56.5	P63261	Actin, cytoplasmic 2	29	0.5099	0.0013	0.92	0.1083
41	P06733	Alpha-enolase Homo sapiens (Human)	9	0.5058	0.0001	0.7502	0.0038
33.3	P07355	Annexin A2	9	0.4459	0.0015	0.8047	0.0188
61.2	P80723	Brain acid soluble protein 1	7	0.523	0.0409	0.7312	0.0264
23.5	P09326	CD48 antigen precursor (B-lymphocyte activation marker  BLAST-1)	5	0.4887	0.005	0.949	0.5258
41.5	O00299	Chloride intracellular channel protein 1	5	0.5008	0.0012	1.0572	0.5918
28	P31146	Coronin-1A	4	0.5112	0.0003	0.8208	0.011
30.1	P68104	Elongation factor 1-alpha 1	8	0.5466	0.0006	0.9394	0.4253
50	P15311	Ezrin (p81)	12	0.5889	<0.0001	0.8771	0.0974
26.3	P04406	Glyceraldehyde-3-phosphate dehydrogenase	3	0.4113	0.0231	0.8528	0.5458
51.2	P11142	Heat shock cognate 71 kDa protein	18	0.5481	0.0042	0.9216	0.5634
29.1	P08238	Heat shock protein HSP 90-beta	8	0.5316	0.003	1.0241	0.8397
46.6	P30453	HLA class I histocompatibility antigen, A-34 alpha chain precursor	14	0.6886	0.0117	0.9655	0.7305
31.1	P01903	HLA class II histocompatibility antigen, DR alpha chain precursor	7	0.4474	0.007	0.9419	0.4304
52.6	P13760	HLA class II histocompatibility antigen, DRB1–4 beta chain precursor	7	0.5259	0.0458	0.9599	0.7407
41.7	P13761	HLA class II histocompatibility antigen, DRB1–7 beta  chain precursor	7	0.5595	0.001	0.9605	0.6705
25.9	P05362	Intercellular adhesion molecule 1 precursor	3	0.5685	0.0489	0.8179	0.1331
57.5	P26038	Moesin	19	0.5428	0.0012	0.9082	0.0732
18.4	Q09666	Neuroblast differentiation-associated protein AHNAK	2	0.4589	0.0143	0.7738	0.025
19.2	P43007	Neutral amino acid transporter A	5	0.3368	0.0098	0.8818	0.5752
32.2	Q06830	Peroxiredoxin-1	5	0.3815	0.0157	0.7814	0.0282
23	P13796	Plastin-2 (L-plastin)	4	0.5221	0.0042	0.8782	0.1899
16.4	Q8WUM4	Programmed cell death 6-interacting protein	2	0.5772	0.0397	0.8987	0.5737
16.2	P30101	Protein disulfide-isomerase A3 precursor	1	0.4702	0.0423	0.7946	0.3432
23.4	P14618	Pyruvate kinase isozymes M1/M2	5	0.5098	>0.0001	0.926	0.2772
26.6	P15153	Ras-related C3 botulinum toxin substrate 2 precursor	2	0.5057	0.0433	0.963	0.811
24.5	P62834	Ras-related protein Rap-1A precursor	1	0.4817	0.0063	0.7928	0.0465
81.8	P62328	Thymosin beta-4	3	0.4805	0.0215	1.0323	0.7312
31.6	P61586	Transforming protein RhoA precursor	2	0.4166	0.0123	0.9393	0.5786
37.1	P67936	Tropomyosin alpha-4 chain	3	0.506	0.0248	0.8225	0.3176
17.6	Q9BQE3	Tubulin alpha-6 chain	3	0.4275	0.0074	0.8795	0.2247
72.4	P62988	Ubiquitin	3	0.4191	0.0037	0.9396	0.5418