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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Neurochem. 2014 Mar 27;130(1):41–49. doi: 10.1111/jnc.12710

Fig. 2.

Fig. 2

Blockade of KCa3.1 channel attenuates TGF-β-induced GFAP protein expression. (A) Representative immunocytochemistry showing GFAP expression in cultured astrocytes treated with TGF-β (10 ng/ml) for 5 days in the presence of TRAM-34 (1, 10μM). Scale bar = 50 μm. Con: control. (B) Representative western blot showing GFAP expression in cultured astrocytes treated with TGF-β (10 ng/ml) for 5 days in the presence of TRAM-34 (1, 10μM). Quantification of western for GFAP immunoreactivity (n = 3). Data are presented as means ± SEM. #p < 0.05 versus control, **p < 0.01, ***p < 0.001 versus TGF-β alone.