Blockade of KCa3.1 inhibits TGF-β-induced CSPG expression. (A) Representative western blot showing CSPG expression in conditioned medium (CM) harvested from astrocytes treated with TGF-β (10 ng/ml) for 5 days in the presence of TRAM-34 (1, 10μM). (B) Representative western blot showing CSPG expression in cell lysates (LY) harvested from astrocytes treated with TGF-β (10 ng/ml) for 5 days in the presence of TRAM-34 (1, 10μM). Quantification of western blot for CS-56 immunoreactivity (n = 3). Data are presented as means ± SEM. ##
p < 0.01, ###
p < 0.001 versus control, * p < 0.05, ** p < 0.01, *** p < 0.001 versus TGF-β alone. Con: control.