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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Neurochem. 2014 Mar 27;130(1):41–49. doi: 10.1111/jnc.12710

Fig. 7.

Fig. 7

Involvement of KCa3.1 in UTP-induced intracellular calcium increase in astrocytes. Astrocytes were loaded with the Ca2+-sensitive dye Fluo-4 AM at 37°C for 30 min and changes in [Ca2+]i were monitored by confocal microscopy. (A) Representative fields of cells treated with UTP or TRAM-34 plus UTP. (B) Changes in [Ca2+]i as averages in changes of fluorescent intensities. 300 μM UTP, a metabotropic purinergic receptor agonist, was added to astrocytes at 60 s. In the presence of 1 μM TRAM-34, UTP caused rapidly decaying Ca2+ influx. Signal acquisition lasted for 1000 s. Data are presented as means ± SEM (n = 20 cells).