mRNA and protein expression of Oatp1a1 (A), Oatp1a4 (B), Oatp1b2 (C), and Oatp2b1 (D). Representative western blots and the Two-way ANOVA p-values are shown. *indicates a p-value <0.05 according to a Two-way ANOVA Bonferroni multiple comparison post-test. Data represent mean ± SEM. WT control n=4, WT NASH n=4, Slco1b2−/− control n=4, Slco1b2−/− NASH n=4. (E) OATP1B1, OATP1B3, and OATP2B1 mRNA expression levels in normal (n=20), steatosis (n=10), and NASH (n=19) livers. (F) OATP1B1, OATP1B3, and OATP2B1 protein expression levels in normal (n=10), steatosis (n=4), and NASH (n=12) livers. Box and whisker plots represent the median and the min and max. *indicates a p-value <0.05 according to a One-way ANOVA Dunnett’s multiple comparison post-test. Representative human OATP blots are shown on the right.