Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Immunol. 2014 Jun 4;193(1):258–267. doi: 10.4049/jimmunol.1400322

Figure 1.

Figure 1

Mice with mutations in the CD3ε-BRS exhibit major deficiencies in thymic development.

(A) Rag1−/− mice reconstituted with bone marrow utilizing mutant CD3ε chains have reduced thymic cellularity (n=26-40 mice per group, at least 5 separate experiments).

(B) Abnormal frequencies of DN and DP thymocytes in mice expressing mutant CD3ε chains (n=21-34 mice per group, at least 5 separate experiments). Statistical significance was determined for all groups at the DN stage and for m2 and m3 at the DP stage when compared to the WT group.

(C) After gating on lineage negative thymocytes (B220, γδ TCR, Gr1, pan NK, CD11c, CD11b, Ter119, CD4, CD8 negative) early thymocyte stages of development were analyzed. Mice utilizing mutant CD3ε chains demonstrate a block at the DN3-DN4 transition (n=23-29 mice per group, at least 5 separate experiments). Statistical Analysis was performed using Mann Whitney t-test. *p<0.05, **p<0.01 ***p<0.001.