Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Immunol. 2014 Jun 6;193(1):354–363. doi: 10.4049/jimmunol.1302999

FIGURE 5. Intrinsic and extrinsic influences on NK cell responsiveness for IL-10 and IFN-γ expression.

FIGURE 5

Donor splenic leukocytes from B6 IL-10-GFP (CD45.2) mice, d 0 or infected with high dose MCMV for 1 or 3 d, were transferred into B6 (CD45.1) recipients that were either d 0, or infected with lose dose virus for 1 or 3 d. Recipient mice were left for one day before harvesting and analyzing IL-10-GFP (A) or IFN-γ (B) expression within gated donor (CD45.2) NK cells. Filled gray histograms indicate basal cytokine expression in transferred d 0 cells into d 0 mice. Blue, green, and red histograms indicate expression in donor NK cells prepared from d 0, d 1-infected, and d 3-infected IL-10-GFP mice, respectively. Summary data are from three experiments, with 4–6 mice per group total. (C) As in A and B with the modification that donor leukocytes from d 3-infected IL-10-GFP mice were adoptively transferred into d 1 B6 recipients that had received 750 ug α-IL-12p40 or control Ab 4 h before infection. Results are from two experiments, 5 mice per group total. (A, B) Numbers on histograms are proportion of cells within gate. Representative data are shown. Results are summarized with symbols representing individual mice. Statistical significances are shown, **P<0.01, ***P<0.0001. For the Figs. 5A summary data, the results are also significant by one-way ANOVA with ***P<0.0001.