Table 1.

Sequences for quantitative RT-PCR and nested-PCR primers and flourescent probes

	Forward primer	Reverse primer	Length (bp)
TaqMan primer CK-19	5'-GAAGGCCTGAAGGAAGAGCTG-3'	5'-CCTCCCACTTGGCCCCT-3'	80
TaqMan primer beta-actin	5'-TCCAGAGGCGCTCTTCCA-3'	5'-CGCACTTCATGATCGAGTTGA-3'	86
Vector primer CK-19	5'-AACTCCAGGATTGTCCTGCAG-3'	5'-TCCCGGTTCAATTCTTCAGTC-3'	401
Vector primer beta-actin	5'-GCACCACTGGCATTGTCATG-3'	5'-CCACACGGAGTACTTGCGC-3'	581
Nested-PCR primer CK-19	5'-AACTCCAGGATTGTCCTGCAG-3'	5'-TCCCGGTTCAATTCTTCAGTC-3'	401
Nested-PCR primer CK-19	5'-GAAGGCCTGAAGGAAGAGCTG-3'	5'-CCTCCCACTTGGCCCCT-3'	80
Probe CK-19	5'-CCTACCTGAAGAAGAACCATGAGGAGGAAATCAGTA-3'
Probe beta-actin	5’-CCTCCTTCCTGGGCATGGAGTCCTG-3’