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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Clin Immunol. 2014 Mar 1;152(0):77–89. doi: 10.1016/j.clim.2014.02.010

Figure 2.

Figure 2

Heavy-chain dendrograms of the plasmablast antibody repertoires of S. aureus-bacteremic individuals and shared HC V-J analysis. Each peripheral node depicts a sequenced VH region with a unique plate and well-ID barcode combination. Plasmablast antibody repertoires of two individuals with S. aureus bacteremia: (A) patient 1 and (B) patient 2. Red lines indicates the IGHV families with greater than 5 recovered full-length VH sequences, from which analysis of shared IGHJ was performed. Pie charts of the IGHJ gene usage for each IGHV family are displayed adjacent to the branch representing each IGHV. For presentation, red bolded lines indicate the IGHV families where a prominent IGHJ gene was used. S1, S2, S3, S4, S5, S6, S7, S8, S9, and S10 denote the recombinant antibodies that were selected for recombinant expression. Circles denote antibodies that bind S. aureus, and squares denote antibodies that do not. Recombinant antibodies from shared gene families are denoted in blue, and antibodies not sharing gene rearrangements are denoted in green.