Table 2.
Results of LTC-IC assay using UCB CD34+ cells or those generated by four culture systems
| Culture systems |
LTC-IC frequency |
Fold LTC-IC amplification | |
| Pre-expansion | Post-expansion | ||
| hMSCs (TPO/FL-free) culture system | 1/619.57 ± 120.89 | 1/102 258.56 ± 9 568.49 | 2.79 ± 0.56 |
| Cytokines culture system | 1/625.38 ± 154.26 | 1/84 112.78 ± 10 867.64 | 3.58 ± 1.48 |
| hMSCs coculture system | 1/621.76 ± 138.59 | 1/93 773.42 ± 20 346.23 | 5.32 ± 1.73 |
| tfhMSCs coculture system | 1/617.43 ± 119.76 | 1/24 140.96 ± 7 586.62 | 10.23 ± 2.89b |
b
P < 0.01 vs calculated by the Student’s t-test, when data of the tfhMSCs coculture system were compared with data in the other groups.