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Effect of internal Ni2+ on the pacemaking activity of ICCs. In order to investigate the effect of NiCl2 on the pacemaking activity of ICCs, we added 20 µmol/L of NiCl2 to the internal solution. A: Under a voltage clamp at a holding potential of -60 mV, the pacemaking activity of ICCs showed a “wax and wane” phenomenon (n = 6). B: Current clamp mode (I = 0) showed the same phenomenon (n = 3).
