Figure 4.

Effects of IFNs on [³H]-proline incorporation in PSCs. PSCs growing in 12-well plates (one passage) were exposed for 48 h to IFNs as indicated. Collagen synthesis was analyzed through the quantification of [³H]-proline incorporation into acetic acid-soluble proteins, and raw data were normalized for differences in cell growth rates as described under “Materials and methods”. One hundred percent [³H]-proline incorporation corresponds to untreated PSCs. Data are presented as mean ± SE (n = 6 separate cell preparations from one rat). ^aP < 0.05 vs control cultures. The results shown are representative of three experiments using cells from different rats.