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Non-isotopic PCR-SSCP analysis of MYH. For detection of sequence alterations, all of the 16 exons and flanking intronic sequences were amplified by PCR as 14 separate fragments, and 20 μL of PCR product was subjected to non-isotopic SSCP analysis. Genomic DNA isolated from the healthy controls was used as normal controls. None of patient samples showed abnormal migration shift of single strand DNA molecules. Lane N: Normal control; Lanes 1-7: Patients; Lane E: Exons.
