FIGURE 3.

Caspase-4 contributes to endotoxin-induced proinflammatory cytokine secretion. LPS (5 mg/kg) or PBS were injected i.p., and sera (A) or cortical brain lysates (B) were collected after 6 h to determine cytokine levels (n = 6–8/genotype). For (A), ANOVAs with Bonferroni post hoc test are shown. For (B), Kruskal–Wallis nonparametric one-way ANOVA with pairwise comparisons was performed. (C) LPS (0.2 mg/kg) was injected and sera collected at 1, 2, and 6 h (n = 8–11/genotype). Serum cytokines were quantitated by multiplex ELISA, and Mann–Whitney nonparametric test was used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001.