FIG. 3.

Intracellular proteins can be detected in decellularized lung homogenates. (A) Four representative blots are shown, respectively, for native lungs, lungs sham decellularized with PBS only, and for lungs undergoing full decellularization. Quantitative data are presented as mean±SEM. Significant interactions were identified by one-way analysis of variance with Bonferroni post hoc analysis using Prism software. *p≤0.05, ^#p≤0.001, ^##p≤0.0001, **p≤0.01, n.s., non-significant. (B) Representative immunofluorescent staining for smooth muscle actin and smooth muscle myosin heavy chain. Original magnifications=200×. (C) Direct GFP fluorescence in native and decellularized C57Bl/6 and eGFP-expressing mouse lungs. Original magnifications 200×. PBS, phosphate-buffered saline; SEM, standard error of the mean; eGFP, enhanced green fluorescent protein. Color images available online at www.liebertonline.com/tea