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| Microinjection | Survival of zygotes | Result of genotyping | Ratio of genotype | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Experiment | gRNA | hCas9 | ssODN | 2 cell embryo/injected zygotes | Transferred | Genotyped | Monoallelic substitution (+indel) | Biallelic substitution (+indel) | Indel | WT | Mouse with substituted alleles (%) | Mouse with mutated alleles (%) |
| 1 | 1 & 2 | D10A | 1 & 2 | 144/188 | 144 | 24 | 5 (0) | 0 (0) | 1 | 18 | 5 (20.8) | 6 (25.0) |
| 2 | 2 | WT | 1 & 2 | 116/139 | 90 | 26 | 6 (1) | 3 (1) | 11 | 6 | 9 (34.6) | 20 (76.9) |
